Isolation of deoxyribonucleic acid from mammalian tissues.

نویسندگان

  • K S Kirby
  • E A Cook
چکیده

1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides remained in solution when DNA was precipitated with 2-butoxyethanol in the presence of 0.5m-sodium chloride and 1.5m-sodium benzoate. 4. Ribosomal RNA was removed by precipitation in the presence of 3m-sodium chloride at 0 degrees , when DNA remained soluble.

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عنوان ژورنال:
  • The Biochemical journal

دوره 104 1  شماره 

صفحات  -

تاریخ انتشار 1967